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Collagenase NB 6 from Clostridium histolyticum (GMP Grade)

Cat No.
NATE-1918
Description
Collagenases from Clostridium histolyticum are proteolytic enzymes that cleave peptide bonds in the triple helical collagen molecule of human or animal tissue in situ. For this reason collagenases are widely used for isolation of various cell types by tissue dissociation.
Source
Clostridium histolyticum
Applications
Collagenase NB 6 GMP Grade is suitable for cell isolation from various tissue types intended for clinical applications. If a research or sterile product is required, Collagenase NB 4 Standard Grade or Collagenase NB 5 Sterile Grade, respectively, are recommended. Both products have comparable enzymatic activities to Collagenase NB 6 GMP Grade.
Form
Lyophilized powder
Enzyme Commission Number
EC 3.4.24.3
Activity
≥ 0.100 U/mg
CAS No.
9001-12-1
Molecular Weight
70 000 - 120 000 (collagenases)
Unit Definition
1 U according to Wünsch catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Storage
2 to 8 °C
Synonyms
Collagenase NB 6; Collagenase NB; Collagenase
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Related Protocols
collagenase -Enzymatic Assay Protocol
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