Services

Professional and Cost-Saving Solutions

Enzyme Activity Measurement for Beta-Agarase



Creative Enzymes offers reliable activity measurement for β-agarase. Having tested activities of variety enzymes in the past several years, Creative Enzymes has accumulated extensive research experiences. We constantly implement improvement and optimizations to our in-house test methods, which deliver the enzyme activity results of utmost quality and reproducibility.

Agar is the major polysaccharide in the cell wall components of marine red algae. Agar contains D-galactose and 3,6-anhydro-L-galactose as monomeric units, which are linked by alternative α-1,3- or β-1,4-glycosidic bonds. In the agarolytic system, enzymes acting on agar are classified into two distinct types, α-agarase (EC 3.2.1.58) and β-agarase (EC 3.2.1.81; agarose 4-glycanohydrolase), depending on which glycosidic bond is cleaved by these agarases during depolymerization. α-Agarases cleave α-1,3-linkage of agarose to produce agarooligosaccharides. Specifically, β-agarases hydrolyzed β-1,4-glycosidic bonds producing neoagarooligsaccharides that have various degrees of polymerization (DP). The major products of β-agarases are neoagarohexaose (DP6) and neoagarotetraose (DP4), which are further cleaved to α-neoagarobiose (DP2). The α-neoagarobiose is then split into monomeric sugars by α-neoagarobiose hydrolase, which is subsequently metabolized in the cell.

Enzyme Activity Measurement for Beta-Agarase Figure 1: A scheme of agar digestion by β-agarase.
Reference: Temuujin U, Chi W J, Lee S Y, et al. Applied microbiology and biotechnology, 2011, 92(4): 749-759.

Agarase activities have been found in many bacteria including Microbulbifier, Pseudoalteromonas, Vibrio, and Zobellia. The activities of agarases from these types of bacteria are mainly related with saprophytic or phytopathogenic activity. As the hydrolysis products of agar are proven to have multiple medical or pharmaceutical effects such as prebiotic, antioxidative, antitumor, or skin-moisturizing and whitening effects, agarases have received significant attentions in the biotechnology industry. Recently, marine algae have been demonstrated to be a potential renewable biomass for bioenergy production and hence microbial agarase activities have shed light on the enzymatic saccharification of agar biomass.

Creative Enzymes is proud to offer the most reliable enzymatic activity assay for β-agarase. The activity of β-agarase is assayed by using the dinitrosalicyclic acid (DNS) method to measure the increase in the concentration of reducing sugar. Optical density is read at 520 nm. The assay results are assured of high reliability by our state-of-the-art technology, a superb expert team, and the most advanced instrument. Our passion for pursuing the best services and customer satisfaction in class has made us the most preferred vendor in the global market.

Enzyme Activity Measurement for Beta-Agarase Figure 2: The crystal structure of β-agarase from Zobellia galactanivorans.
PDB: 1O4Y



Inquiry
Related Products
CatalogEXWM-3945
ProductNameβ-agarase
EC No.EC 3.2.1.81
CAS No.37288-57-6
Source
CatalogNATE-1293
EC No.EC 3.2.1.81
CAS No.37288-57-6
SourceE. coli
CatalogNATE-0040
EC No.EC 3.2.1.81
CAS No.37288-57-6
SourcePseudomonas atl...
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country or Region:
Company/Institution:
*Products or Services of Interest:
Quantity:
Project Description:
Our products are not intended for private therapeutic use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.


Sitemap | Privacy Policy | Terms and Conditions
Copyright ©2011 - 2019 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-516-512-3133
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: