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Enzyme Activity Measurement for Gluconate 5-Dehydrogenase Using Spectrophotometric Assays

Creative Enzymes is specialized in enzyme activity measurement, especially for oxidoreductases such as gluconate 5-dehydrogenase. We are proud of running the cutting-edge equipment and earning extensive experiences over years which make us to maintain a leading position in this field.

Gluconate 5-dehydrogenase (EC1.1.1.69; Ga5DH) is an enzyme that catalyzes a reversible oxidoreduction reaction between D-gluconate and 5-keto-D-gluconate, while simultaneously generating NADPH, which acts as a hydrogen donor for many biosynthetic processes. To date, biochemical characterization has been reported for gluconate 5-dehydrogenases from several species, including Gluconobacter oxydans, Gluconobacter liquefaciens, and Acetobacter suboxydans. The enzyme belongs to the short chain dehydrogenase/reductase (SDR) family with a preference for NADP+ rather than NAD+ as cofactor. The systematic name of this enzyme class is D-gluconate: NAD(P)+ 5-oxidoreductase. Other names in common use include:

Gluconate 5-dehydrogenase plays an important role in L-idonate degradation, which is a critical pathway for ketogluconate metabolism. Gluconate 5-dehydrogenase is the second enzyme in the pathway of L-idonate degradation. In this pathway L-idonate is catabolized through the intermediate D-gluconate. L-Idonate is converted to D-gluconate by two consecutive oxidation and reduction reactions which are catalyzed by gluconate 5-dehydrogenase and another enzyme. D-Gluconate is then phosphorylated, forming 6-phosphogluconate which is an intermediate of central carbon metabolism. 6-Phosphogluconate is later metabolized via the pentose phosphate or Enter-Doudoroff pathway. Therefore, accompanied with energy consumption, 5-keto-D-gluconate can be converted to D-gluconate by gluconate 5-dehydrogenaseand and subsequently enter the Entner-Doudoroff pathway to join as a source of carbon and NADP+. However, no structural information for gluconate 5-dehydrogenase is currently available to allow elucidation of the molecular architecture or clarification of the catalytic mechanism of the enzyme. Recently, some studies have shown that gluconate 5-dehydrogenase also seems like an effective target for the development of novel potent antibacterial agents. Despite of the multiple functions of gluconate 5-dehydrogenase in medical, biological, and pharmaceutical industries, the activity assay of this enzyme has not been well developed due to lack of structural information. In the meantime, Creative Enzymes is proud to offer the fast and accurate service of enzyme activity measurement for gluconate 5-dehydrogenase. We have the world’s leading technologies and latest models of UV/vis spectrometer. By constantly striving for products of utmost quality, Creative Enzymes has earned the trust of countless customers. Regardless of the challenges which may lay on your way to enzyme applications, Creative Enzymes will always meet the requirements for the next development stage and will be your best choice with satisfying services.

Enzyme Activity Measurement for Gluconate 5-Dehydrogenase Using Spectrophotometric Assays
Figure: The crystal structure of gluconate 5-dehydrogase from Streptococcus suis type 2.

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