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Enzyme Activity Measurement for N-Acetyllactosaminide Beta-1,6-N-Acetylglucosaminyl Transferase Using Chromatographic Assays

Creative Enzymes has been serving the enzyme industry for many years. We created exclusive and unique enzyme assays to be distinguished in the global marketplace. Equipped with the most advanced instruments, Creative Enzymes is able to offer the first-class technical support and personalized customer services. Hereupon, Creative Enzymes is enthusiastic to offer the reliable activity assay for N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase.

N-Acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase (EC 2.4.1.150) is an enzyme that catalyzes the chemical reaction converting UDP-N-acetyl-D-glucosamine and beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide to UDP and N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide. This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. N-Acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase is a branching enzyme that turn linear poly N-acetyllactosaminoglycans into branched ones. The enzyme was initially identified in microsomal preparations from hog gastric mucosa. A cDNA of the human enzyme was first isolated in 1993 and expressed in Chinese hamster ovary (CHO) cells.

The enzyme from mammals introduces the blood group I antigen during embryonic development. The I antigen is found on membrane proteins of the red blood cells and some other cell types, as well as different body secretions of developing fetuses and newborn infants, while the I antigen is found in all adults. The i antigen undergoes gradual conversion to the I antigen in newborn infants. Both antigens are based entirely on Type 2 (N-acetyllactosamine) chains. The basic i structure is an unbranched polylactosamine. Importantly, the key step in the conversion of the i antigen to the I antigen is catalyzed by N-acetyllactosaminide β-1,6-N-acetylglucosaminyl-transferase, which introduces the branching by adding a GlcNAc residue to the galactose. This process is closely associated with the development and maturation of erythroid cells.

Figure: The crystal structure of human N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase. Figure: The crystal structure of human N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase.
UniProt: Q8N0V5

Additionally, N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase plays a pivotal role in multiple pathways, such as glycosphingolipid biosynthesis - lacto and neo lacto series, terminal O-glycans residues modification, and other metabolic pathways. Therefore, N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase is getting more and more attentions because of various functional significance.

Creative Enzymes offers the most advanced chromatographic assay to quantify the enzymatic activity of N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase accurately. The activity measurement of this enzyme is performed using thin layer chromatography (TLC). Services of Creative Enzymes are the most trusted and proven in the global market, resulting in positive feedback and a high rate of repeated sales. Overall, Creative Enzymes is a leading biotech company that partners with diverse and unique customers to support the needs in fundamental research and process optimization.



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Related Products
CatalogEXWM-2377
EC No.EC 2.4.1.150
CAS No.85638-40-0
Source
CatalogNATE-1489
EC No.EC 2.4.1.150
CAS No.85638-40-0
SourceE. coli
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