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Enzyme Activity Measurement of 3-Hydroxybutyrate Dehydrogenase Using Spectrophotometric Assays



Creative Enzymes is specialized in measuring enzyme activities. Having tested activities of variety oxidoreductases in the past few years, Creative Enzymes has accumulated extensive and professional experiences, which can provide rapid and high-quality enzyme activity assay services, including 3-hydroxybutyrate dehydrogenase.

3-Hydroxybutyrate dehydrogenase belongs to the SDR (short-chain dehydrogenase) family. This enzyme catalyzes the transformation from (R)-3-hydroxybutanoate to acetoacetate, using NAD+ as a cofactor. This enzyme also oxidizes other 3-hydroxymonocarboxylic acids. The enzyme is given alternative names as follows:

3-Hydroxybutyrate dehydrogenase was found in rat liver mitochondria, and has been purified from the human heart, bovine heart, and rat liver. It has also been found in many microorganisms. The enzyme has the specific requirement of phosphatidylcholine for activity. In contrast, the bacterial enzymes do not require phospholipids for their activity. This enzyme participates in synthesis and degradation of ketone bodies and butanoate metabolism. So, the enzyme derived from Rhodobacter sp.7 has been used for the diagnostic analysis of ketone bodies for diabetes mellitus, which reveals huge potential of the enzyme as a key diagnosis reagent.

The structure of this enzyme was suggested to be a homotetramer. DL-2-hydroxybutyrate and DL-lactate are effective inhibitors, while, sodium cacodylate also strongly inhibits enzyme activity. Creative Enzymes can handle all complicated situations appeared in your research and give an accurate and reliable result of activity assay. Owing to the fact that 3-hydroxybutyrate dehydrogenase could also catalyze some β-hydroxy acids, including L-3-hydroxybutyrate, L-threonine, D-threonine, L-serine, D-serine, and DL-glycerate substrates, Creative Enzymes also provides the directional or quantitative substrate specificity test according to the customer’s demand.

Enzyme Activity Measurement of 3-Hydroxybutyrate Dehydrogenase Using Spectrophotometric Assays
Figure: The crystal structure of D-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi complexed with NAD+.
Reference: Ito K et al. J Mol Biol. 2006 27;355(4):722-33

Creative Enzymes has participated in the industry of enzyme activity measurement for many years. Extensive and professional experiences help us gained a good reputation from customers and researchers. In the future, Creative Enzymes will continue to support your aim at notable achievements with our specialized services.



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