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Enzyme Activity Measurement of Acetoacetyl-CoA Reductase Using Spectrophotometric Assays

Creative enzymes is proficient in enzyme activity assays. With the skilled specialists and advanced instruments, Creative Enzymes has set up the new standard in enzyme assay industry. We provide the most reliable testing result for oxidoreductase, including acetoacetyl-CoA reductase.

Acetoacetyl-CoA reductase belongs to the family of oxidoreductases. It catalyzes the oxidation reaction of (R)-3-hydroxyacyl-CoA to 3-oxoacyl-CoA, using NADP+ as cofactor. The alternative names are as follows:

The enzyme was found contributing to the synthesis of polyhydroxybutyrate (PHB) from acetyl coenzyme A (acetyl-CoA) in bacterium Ralstonia eutropha H16, accompanied with other enzymes. Polyhydroxybutyrate (PHB) is one of polyhydroxyalkanoate (PHA), a polymer belonging to the polyesters class. These materials are of strong interest as bio-derived and biodegradable plastics. Polyhydroxyalkanoates (PHAs) are naturally occurred polyesters that are produced by a wide range of bacteria and used as carbon and energy reserves. PHAs are typically stored when organisms are in an environment that is rich in carbon sources but in lack of another nutrient, limiting normal cell growth. Particularly, under the conditions with fluctuating carbon levels, PHA producers have been found to show crucial advantages over rival species. In addition to their importance in the microbial world, these polymers have been studied for their potential uses in biodegradable consumer goods and medical products, and as chemical precursors. As a key enzyme in PHB synthesis, as well as a functional enzyme in butanoate metabolism, acetoacetyl-CoA reductase has been widely studied by the chemical industry for sustainable and environmental friendly processes. The increasing needs in activity testing stimulate great excitement. Creative Enzymes provides quality and quantity assured testing services for acetoacetyl-CoA reductase to support research in an efficient and accurate way.

Enzyme Activity Measurement of Acetoacetyl-CoA Reductase Using Spectrophotometric Assays
Figure: The crystal structure of a putative acetoacetyl-CoA reductase from Burkholderia cenocepacia bound to the cofactor NADP.

Based on the professional experts and skilled techniques, Creative Enzymes has become the leading service company in providing standard and efficient enzymatic activity measurement. The unparalleled services and reliable tests will support you towards notable achievements.

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