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Enzyme Activity Measurement of Carbon-monoxide Dehydrogenase (Ferredoxin) Using Spectrophotometric Assays



Creative Enzymes makes every effort toward providing the best enzyme activity testing with attractive cost performance ratios. Our unparalleled services are labelled with high accuracy and reliability. We are the expert in oxidoreductase activity measurement, such as carbon-monoxide dehydrogenase (ferredoxin).

Carbon-monoxide dehydrogenase (ferredoxin), also named anaerobic carbon-monoxide dehydrogenase and Ni-CODH, is of great importance in the global carbon cycle. In prokaryotes, this enzyme catalyzes the reversible reduction of CO2 to CO, and, simultaneously, the electrons are transferred to redox proteins such as ferredoxin. In purple sulfur bacteria and methanogenic archaea, it catalyzes the oxidation of CO to CO2 incorporated by the Calvin-Benson-Basham cycle, or released in the reverse reaction. In acetogenic bacteria, including well-characterized Moorella thermoacetica, CODH-catalyzed CO2 reduction is coupled with acetyl-CoA synthesis in the bifunctional enzyme complex CODH/acetyl-CoA synthase (ACS) as part of the Wood−Ljungdahl carbon fixation pathway.

All carbon monoxide dehydrogenases (CODHs) characterized so far are homodimeric and contain five metalloclusters: two NiFeS C-clusters buried in the active site, two Fe4S4 B-clusters, and one Fe4S4 D-cluster located at the dimeric interface near the surface of the enzyme. Studies have shown that Ni is part of a distorted cubane-like NiFe3S4 cluster, and an additional, unique Fe, often referred to as ferrous component II (FCII), is connected to a sulfide of the cubane.

Ever since the carbon-monoxide dehydrogenase (ferredoxin) was discovered has the widespread research interest been aroused. In the past decade, the studies on structures and functions gained great progresses, owing to its significance in environment protection. However, the measurement of enzyme activity is a tough task during research, because the enzyme activity can be easily inhibited by oxygen. Often during an activity test, exposure to air can cause a sharp decrease in enzyme activity. For example, carbon-monoxide dehydrogenase (ferredoxin) from Methanosarcina barkeri is strongly inhibited by and extremely sensitive to oxygen. The enzyme loses activity in less than 1 min once exposed to air. In order to minimize the impact of the oxygen, Creative Enzymes provide testing services operated with advanced devices to protect enzymes from oxygen during the assay process.

Enzyme Activity Measurement of Carbon-monoxide Dehydrogenase (ferredoxin) Using Spectrophotometric Assays
Figure: The native structure of bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase from Moorella thermoacetica, water-bound C-cluster.
Reference: Yan Kung et al. Biochemistry. 2009 48(31): 7432–7440

Creative Enzymes has the capability of formulating more targeted plans for different enzyme activity assays. Specialized equipment are used to satisfy your unique demands on enzyme activity quantification. We promised and delivered high-quality services and accurate results in the past several years. Our constantly growing business and positive feedback prove your wise choice of Creative Enzymes.



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CatalogEXWM-1227
EC No.EC 1.2.7.4
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