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Enzyme Activity Measurement of Fructose 5-Dehydrogenase

Creative Enzymes offers the top-quality assay service for enzyme activity measurement, relying on our professional research team and most advanced equipment. Our results are guaranteed with high reproducibility and accuracy. We are specialized in testing oxidoreductase such as fructose 5-dehydrogenase.

Fructose 5-dehydrogenase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with other acceptors. This enzyme catalyzes the conversion form D-fructose towards 5-dehydro-D-fructose, using other chemicals as electron acceptors, such as 2,6-dichloroindophenol. However, oxygen is completely inactive as an electron acceptor. Up till now, this enzyme has only been found in Gluconobacter sp., but in different strains, this enzyme uses different cofactors to achieve its catalyze activity, such as cytochrome c, FAD, heme, heme c, and pyrroloquinoline quinone. There are some other names in use including:

Although the exactly structure information about fructose 5-dehydrogenase remains unknown except the trimer subunits, the application of this enzyme becomes more popular recently. For example, it has been used in diagnosis and food analysis, because of its strict substrate specificity. This enzyme is also used in examining the electrochemical properties of enzyme-catalyzed electrode reactions in a number of basic research projects, which are called bioelectrocatalysis. As far as we know, fructose 5-dehydrogenase has the strong and the highest ability of direct electron transfer (DET)-type biocatalysis on the anode, which is convenient for the construction of compact bioelectrochemical devices. It is utilized to develop biosensors, biofuelcells, and bioreactors. Although it is attractive for applications, the mechanisms for the enzymatic reaction have not been fully described yet. Nowadays, thousands of researchers started studying the mechanism and function of this enzyme. The efficient and correct approach towards enzyme activity testing is an urgent demand. With all considered, Creative Enzymes offers the best and accurate testing service for fructose 5-dehydrogenase.

Creative Enzymes accomplishes enzyme assays with good performance and high-quality. The professional standard and efficient services are approved by many customers. In the future, Creative Enzymes will continue providing technical support for your research.

Figure: The reaction catalyzed by fructose  5-dehydrogenase. Figure: The reaction catalyzed by fructose 5-dehydrogenase.

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