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Enzyme Activity Measurement of Glucan 1,3-Beta-Glucosidase

Creative Enzymes redefines the enzyme activity assays by the high efficient test services. The supreme service quality is endorsed by tens of thousands of customers. The professional attitude and excellent operation not only contribute to the reliable service but also support the accuracy of results. Here, we are proud to offer activity assay service for glucan 1,3-beta-glucosidase.

Glucan 1,3-beta-glucosidase (EC 3.2.1.58) belongs to the glycoside hydrolase family 3, which is an enzyme family containing a great number of enzymes that hydrolyze the glycosidic bond between two carbohydrates. Glucan 1,3-beta-glucosidase successively removes single glucose units from the nonreducing ends of polymeric and oligomeric beta-glucans, with retention of anomeric configurations.

Existing in higher plants, fungi, and bacteria, the family 3 glycoside hydrolases are thought to play key roles in fundamental biological processes such as the normal growth and development of plant cells, the modification of carbohydrate structure in changing environmental conditions or in the face of pathogen attack, and the microbial degradation of plant residues. As for glucan 1,3-beta-glucosidases, many applications have already been developed. They have potential applications in biotechnology such as cell fusion, transformation and protoplast preparation, as well as in brewing industries. These enzymes also have clinical significance because they are the major immunodominant antigen of pythium insidiosum, causative agent of pythiosis, a life-threatening infectious disease of humans and animals in tropical and subtropical areas. Therefore, they become a research target for drug development. Creative Enzymes is one of the few companies that provide activity assay service for glucan 1,3-beta-glucosidase. The enzyme activity is measured by determining the reducing sugar content with the most reliable spectrophotometric assays. Our professional experts can customize and provide the most targeted approaches to meet particular research demands.

Enzyme Activity Measurement of Glucan 1,3-Beta-Glucosidase Figure: The crystal structure of beta-1,3-glucanase from Phanerochaete chrysosporium.
Reference: Ishida, T. et al. J Biol Chem. 2009 284(15): 10100-10109.

Creative Enzymes is specialized in hydrolases activity testing. We have the best-performed spectrophotometer in the research center, which to a great extent guarantees the reliability and reproducibility of the outputs. Standing on the foundation of in-depth studies of enzymology, Creative Enzymes will continue progressing to further support your research.



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