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Enzyme Activity Measurement of Glucose 1-Dehydrogenase [NAD(P)+] Using Spectrophotometric Assays



Creative Enzymes provides the first-in-class services for enzyme activity assays. Built on the foundation of extensive experiences and cutting-edge technologies, our test approaches are the most suitable and give the most accurate results. We are specialized in testing oxidoreductases, including glucose 1-dehydrogenase [NAD(P)+].

Glucose 1-dehydrogenase [NAD(P)+] (EC 1.1.1.47) belongs to the family of oxidoreductases, it catalyzes the oxidation reaction of β-D-glucose to produce D-glucono-1,5-lactone, using NAD or NADP as the cofactor. Studies have shown that this enzyme has similar activity levels using either NAD+ or NADP+. This enzyme is also known as:

Glucose 1-dehydrogenase [NAD(P)+] is related to the Entner-Doudoroff pathway. This pathway exists in some obligate anaerobic microorganisms, taking the role of the glycolysis pathway to generate pyruvate. Glucose 1-dehydrogenase [NAD(P)+] is the first enzyme in this pathway, catalyzing the oxidation of glucose into D-glucono-1,5-lactone. However, it has been shown that this enzyme has activity with a broad range of sugar substrates, including glucose, galactose, xylose, and L-arabinose, with specific requirement for the stereo configuration at the C2 and C3 positions of the sugar substrate. EC 1.1.1.47 from Bacillus has been deeply studied. It is a tetrameric enzyme with four identical subunits. The enzyme is inactivated in alkaline solutions, and the inactivation is due to the reversible dissociation of the tetramer into inactive protomers. Other sources of glucose 1-dehydrogenase [NAD(P)+] are found belonging to the medium chain alcohol dehydrogenase (MDR) superfamily, which needs zinc ions during the catalysis progress. The activity of these enzymes can be determined spectrophotometrically by following the increase in absorbance at 340 nm, corresponding to the reduction of NAD or NADP. Creative Enzymes is able to perform accurate assays for this enzyme in various solutions. Our testing results are highly reliable and reproducible.

Enzyme Activity Measurement of Glucose 1-Dehydrogenase [NAD(P)+] Using Spectrophotometric Assays Figure: The structure of glucose 1-dehydrogenase [NAD(P)+] from Sulfolobus solfataricus in complex with NADP.
Reference: Milburn CC et al. J Biol Chem. 2006 281(21):14796-804

Creative Enzymes is particularly professional in enzyme activity measurement. Having tested thousands of enzymats, Creative Enzymes obtained extensive experiences to assure the assay service with high accuracy. Our advanced equipment and knowledgeable specialists will support your research in a rapid and efficient way. In the future, Creative Enzymes will continue to be your trustworthy partner. 



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