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Enzyme Activity Measurement of Glycerol Dehydrogenase Using Spectrophotometric Assays

Creative Enzymes provides fast and accurate service of enzyme activity measurement, especially measurement for oxidoreductases. EC catalyzes the chemical reaction using NAD as the electron acceptor, which is the main feature of oxidoreductases. Creative Enzymes is an expert in this filed because of our professional experiences.

Glycerol dehydrogenase (EC, also known as glycerin dehydrogenase, NAD+-linked glycerol dehydrogenase, glycerol: NAD+ 2-oxidoreductase, GDH, GlDH, and GlyDH) is an enzyme in the oxidoreductase family that utilizes NAD+ to catalyze the oxidation of glycerol to form glycerone (dihydroxyacetone). This enzyme belongs to the family of oxidoreductase and, more specifically, a metal-dependent alcohol dehydrogenase. The enzyme plays an important role in anaerobic glycerol metabolism. It has been isolated from a number of bacteria, including Enterobacter aerogenes, Bacillus megaterium and so on. Glycerol dehydrogenase bears a global structure as a homooctamer, which is composed of eight identical monomer subunits made up of a single polypeptide chain containing 370 amino acids. Each subunit two distinct domains (N-terminal and C-terminal), which form the active site of the enzyme in a shape of a deep cleft between these two domains. This active site consists of one bound metal ion, one NAD+ nicotinamide ring binding site, and a substrate binding site.

Figure: The structure of glycerol dehydrogenase from B. stearothermophilus in a complex with glycerol.

EC acts on the CH-OH group of the substrate glycerol, or, the donor, with NAD+ or NADP+ as the acceptor. The measurement of enzyme activity is commonly based on spectrophotometry, which detects the consumption of NAD+ at 260 nm or the generation of NADH at 340 nm. Creative Enzymes presents the particular activity assay for glycerol dehydrogenases, which is simple, sensitive and rapid and can detect low concentrations of glycerol dehydrogenase.

Creative Enzymes is your best choice for glycerol dehydrogenase activity assays. We can measure glycerol dehydrogenase activity no matter what obstruction exists in the solvent. Our research center stands out with advanced equipment, rich experiences and a professional team, which will achieve high efficiency and accuracy for the activity measurement. Overall, Creative Enzymes will use our unique advantages to become your best working partner and support your research.

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