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Enzyme Activity Measurement of Glyoxylate Reductase Using Spectrophotometric Assays

Creative Enzymes is the expert in enzyme activity measurement. We have not only professional techniques and extensive experiences but also advanced equipment and superb teams. All of these in Creative Enzymes will assist our customers in conducting the measurement of glyoxylate reductase activity.

Glyoxylate reductase (EC 1.1.1.26) was first isolated from spinach leaves. The alternative names of this enzyme are as follows:

  • NADH-glyoxylate reductase
  • glyoxylic acid reductase
  • NADH-dependent glyoxylate reductase

Glyoxylate reductase is an important enzyme involved in the glyoxylate metabolism. It has been found in various organisms, including archaea, bacteria, yeast, algae, plants and human. For the plant, glyoxylate reductase helps to keep plant cells healthy and detoxifies the cell by reducing glyoxylate levels. In human, glyoxylate reductase/hydroxypyruvate reductase (hGRHPR) functionally converts the metabolic byproduct glyoxylate into the less reactive glycolate. In biopharmaceutical engineering, glyoxylate reductase also plays an important role, for it provides an opportunity for the synthesis of chiral hydroxy acids, which are of biotechnological interest as an intermediate for the synthesis of various pharmaceuticals.

Enzyme Activity Measurement of Glyoxylate Reductase Using Spectrophotometric Assays Figure: Structure of a glyoxylate reductase from Paecilomyes thermophile.
Reference: Duan X, et al. Enzyme Microb Technol. 2014 10;60:72-9

Glyoxylate reductase belongs to the d-2-hydroxyacid dehydrogenase family. This enzyme catalyzes the reduction of glyoxylate to glycolate. Many glyoxylate reductases also catalyze the interconversion between hydroxypyruvate and d-glycerate. It can use both NADPH and NADH as a cofactor, but prefers NADPH. The oxidation of NADPH can be monitored spectrophotometrically at 340 nm. To meet the increasing demands of glyoxylate reductase activity assay, Creative Enzymes provides glyoxylate reductase activity measuring not just for purified native and recombinant enzymes, moreover, for extracts containing this enzyme.

Creative Enzymes possesses superb technology, professional knowledge, and advanced equipment that can support your research in an excellent way. Our high-quality enzyme assay service has been commended by more and more customers and scientists. In the future, Creative Enzymes promises sustaining the professional standard and efficient services to continue to be your trustworthy partner.