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Enzyme Activity Measurement of L-Amino-Acid Oxidase

Creative Enzymes is one of the top service providers for enzyme activity measurement. We use cutting-edge techniques to achieve high-quality services with rapid turnover and high accuracy. We are especially professional in testing oxidoreductase such as L-amino acid oxidase.

L-Amino acid oxidase (LAAO) is a flavoenzyme that catalyzes the deamination of L-amino acids to the corresponding α-keto acids with the production of ammonia (NH3) and hydrogen peroxide (H2O2), simultaneously accompanied with the consumption of H2O. This enzyme was first found present in snake venoms, and subsequently found to exist in algae, bacteria, actinomycete, and fungi. L-amino acid oxidases vary between genera and species, and snake venom LAAOs are best characterized. The catalysis process requires FAD as a cofactor. Structure studies showed that the FAD-binding domain is highly conserved in both prokaryotes and eukaryotes. L-amino acid oxidases have a wide range of substrate specificity: a majority of snake venom sourced LAAOs can act on at least a dozen L-amino acids, while LLAO from Rhodococcus opacus can catalyze 20 L-amino acids. Studies also point out that the thermal stability, optimum pH, molecular weight, and specific activity from different sources are greatly different.

L-Amino acid oxidase is a cytotoxic protein, triggering a wide spectrum of secondary effects such as blood clotting, myotoxicity, neurotoxicity, platelet aggregation, and lipid digestion. L-Amino acid oxidase acts at specific points and interfere with the highly-regulated blood coagulation cascade and platelet aggregation. Therefore, the enzyme has been employed as a diagnostic and clinical tool. With the thorough study of the inhibition mechanism of cell growth and proliferation, a great number of medical research on the induced apoptosis in tumor and cancer has been aroused. Suitable activity methods can push forward the clinical therapy applications of the L-amino acid oxidases, and Creative Enzymes will be a powerful partner in your research, supporting with accurate and precise assay services.

Creative Enzymes developed up-to-date experiment methods using advanced testing devices. We are capable of providing assay service beyond the knowledge of ordinary service providiers. Creative Enzymes enjoys an outstanding reputation will be honored to become your reliable partner.

Figure: The crystal structure of L-amino acid oxidase from the B. jararacussu venom.
Reference: Ullah, A. et al. Biochem.Biophys.Res.Commun. 2012 421: 124-128   

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