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Enzyme Activity Measurement of Malate Dehydrogenase (Oxaloacetate-Decarboxylating) (NADP+) Using Spectrophotometric Assays

Creative Enzymes is professional in providing accurate and high-quality activity measurement services. Having the most advanced instrument and technical team, Creative Enzymes can perform enzymatic activity testing in a reliable and efficient way, including measurement of oxidoreductases such as malate dehydrogenase (decarboxylating) (NADP+).

Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) belongs to the family of oxidoreductases and catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP+ and divalent metal ions, such as Mg2+ or Mn2+. It can also catalyze the decarboxylation of oxaloacetate which differs from EC Its alternative names are:

Regarding its wide distribution in biological species, malate dehydrogenase (decarboxylating) (NADP+) exists in a broad range of eukaryotic organisms, from fungi to mammals. Malic enzymes are the core component of many important biological functions. In plant, NADP-malic enzyme is one of the three decarboxylation enzymes used in the inorganic carbon concentrating mechanisms of C4 and CAM plants. In fungi and other organisms, a malic enzyme, via NADPH production, is believed to have lipogenic functions. Similarly, the cytosolic NADP+-dependent isoform of malic enzyme (cNADP-ME) in mammals is involved in the generation of NADPH and the biosynthesis of fatty acids and steroids in liver and adipose tissues. In addition, cNADP-ME may also have a role in microsomal drug detoxification. More and more researches on malate dehydrogenase (decarboxylating) (NADP+) have been initiated due to the pivotal position it holds in biology. The increasing needs of accurate enzyme activity assays become a gap in biotechnology to be solved. To this end, Creative Enzymes brings the reliable spectrophotometric assay services to support research and development activities on it.

Enzyme Activity Measurement of Malate Dehydrogenase (Oxaloacetate-Decarboxylating) (NADP+) Using Spectrophotometric Assays Figure: The crystal structure of human NADP-dependent malic enzyme.

Creative Enzymes in the leading service company in the enzyme assay industry. The advanced equipment and experienced specialists here will support your research in a timely and effective way. In the future, Creative Enzymes will continue to be the cornerstone of your success.

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SourceChicken liver
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