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Enzyme Activity Measurement of Polynucleotide 5'-Hydroxyl-kinase Using Spectrophotometric Assays

The extraordinary performance in enzyme activity testing distinguishes Creative Enzymes from other suppliers. Our assay platforms include all types of main analytical instruments. Our professional reputation on activity testing stems from a team of specialized enzymologists. As experts, we provide activity assays for transferases, such as 5’-polynucleotide hydroxyl-kinase.

Polynucleotide 5'-hydroxyl-kinase (PNK) can be found in viruses and cellular organisms. The most widely exploited function of PNK is its ability to catalyze the transfer of the γ-phosphate of a nucleoside triphosphate to the 5’-hydroxyl of a polynucleotide. The presence of Mg2+ is essential to the kinase activity of PNK.

Polynucleotide 5'-hydroxyl-kinase (PNK) carries a critical role due to the indispensable function in various DNA repair processes. In human cells, RNAi knockdown of PNK leads to marked sensitization to spontaneous mutation, as well as to various genotoxic agents. Studies have shown that the mutations in the PNK phosphatase domain are associated with the developmental neurological disorder. Clinical manifestations are microcephaly and refractory epilepsy. Therefore, this enzyme is identified as closely related to human neurological development. As a DNA repair protein, PNK has greatly aroused clinical attentions. By inhibiting the activity of this protein, the sensitivity of tumor cells to radiotherapy and chemotherapy can be improved, thus providing a new strategy to the cancer treatment. Noticing the huge potentials in clinical research, an increasing number of studies are being carried on this enzyme. Activity researches based on the site-directed mutations are the major approaches to locate the enzyme active sites. Therefore, activity measurement must be precise and reliable. To this end, Creative Enzymes offers suitable activity assays which can minimize interferences from other enzymes or additives.

Figure: The crystal structure of polynucleotide  5'-hydroxyl-kinase from mouse.
Figure: The crystal structure of polynucleotide 5'-hydroxyl-kinase from mouse.
Reference: Garces F et al. Mol Cell. 2011 44(3): 385-396.

Creative Enzymes is one of the few companies that provide precise activity assays for polynucleotide 5'-hydroxyl-kinase. Different from kinase activity assay kit, our method is more targeted and reproducible. The measurement is guaranteed to be completed in a limit time with reliable results. In the future, Creative Enzymes will stay as your trust-worthy partner.



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