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Enzyme Activity Measurement of Triacylglycerol Lipase

Creative Enzymes exceeds in hydrolases activity testing. Through the in-depth study of enzymology, our experts bring the most suitable method for triacylglycerol lipase to meet different demands. The results are given with the smart chromatograph, which guarantees precision and accuracy

Triacylglycerol lipase (EC 3.1.1.3) plays a key role in dietary fat absorption in the intestine. This enzyme converts insoluble long-chain triacylglycerol into diacylglycerol and carboxylate. The smaller and more polar product molecules are able to cross the enterocytes membrane when mixed with bile salts. Crystal structures of the human lipases have shown that the polypeptide chain is divided into two domains bearing specific functions. The N-terminal domain contains the catalytic triad and is responsible for triglyceride hydrolysis, whereas the C-terminal domain is involved in colipase binding.

Besides the role in triglyceride digestion through lipid emulsification and intestinal fat absorption, triacylglycerol lipase has various applications. In biotechnology, the enzyme can be used for hydrolysis and synthesis of various esters, mutagenic modification, and properties optimization. In biofuel production, this enzyme acts on degummed soybean oil, synthesizing lipase biodiesel. In food industry, it contributes to the improvement of food texture, flavor modification, and the production of fatty acids. Triacylglycerol lipase can also be used to remove fatty stains under conditions of a modern machine wash and in the alkaline environment. The extensive applications of this enzyme trigger an increasing number of related studies. However, because it acts only on an ester-water interface, ions, salts, and surfactants are often used to facilitate the enzymatic reaction and stabilize the enzyme. Thus, there may be interferences from many ingredients in the reaction mixture when testing the enzyme activity. Fully considering the influences from various factors, Creative Enzymes chooses the most suitable method to perform activity quantification by chromatographic assays.

The crystal structure of the pancreatic lipase-colipase complex Figure: The crystal structure of the pancreatic lipase-colipase complex.
Reference: van Tilbeurgh, H. et al. Nature 1992 359: 159-162.

The chromatographic assay is the most reliable method to achieve precise quantification of enzyme activity. With both the experienced enzymologists and the advanced equipment of Creative Enzymes, the results are of high reproducibility and less variations. In the future, Creative Enzymes will continue to be a strong supporter for your research.



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