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Enzyme Expression in Transgenic Animals



Creative Enzymes provides custom enzyme expression services and delivers high-quality enzymes using the cutting-edge technology of recombinant DNA expression to help researchers and companies to produce desired recombinant enzymes efficiently. Our customers appreciate the fast delivery of interested enzymes and optimization of their own enzyme production workflow. Our specialized capability makes various expression systems available to fulfill the expression and production needs of diverse clients. Especially, Creative Enzymes is glad to highlight our Transgenic Animals Expression System to our clients.

When choosing the ideal expression system, production yield and efficiency, the quality and functionality of the enzymes, and production cost are the most important factors to consider. Transgenic animals are popular in producing recombinant enzymes that have complex structures and post-translational modifications. Enzymes can be produced in milk, egg white, blood, urine, seminal plasma, and silk worm cocoons of transgenic animals. Up till now, milk and urine are demonstrated to be the best carrier of recombinant enzymes. In general, transgenic animals such as goats, mice, cows, pigs, rabbits, and sheep are being developed as production systems, meanwhile some aquatic animals are also being utilized. Foreign proteins can be produced in the mammary glands of these transgenic animals. Compared with other systems of expression and production, transgenic animals system is overall an attractive platform because transgenic animals represent a powerful tool to address the growing need for therapeutic recombinant proteins. More specifically, transgenic animals are able to produce complex, highly active enzymes in an efficient and economic manner that is superior to bacteria, mammalian cells, transgenic plants, and insects. In fact, bacteria are limited in their ability to perform the post-translational modifications necessary for many enzymes, and transgenic plants and insect cells expression systems include relatively slow production setups and have yet to cross many regulatory hurdles.

Selection of a suitable method for recombinant enzyme expression is dependent on the characteristics and intended application of the recombinant enzyme. After years of development, milk is so far the most mature system for producing recombinant enzymes from transgenic organisms. Blood, egg white, seminal plasma, silk gland, and urine are also used in other cases. With all factors considered, each expression system has its own advantages and disadvantages.

Factors to consider Milk Blood Egg white Seminal fluid Urine Silk cocoon
Production level

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Investment cost

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Production cost

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Sacling-up

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Collection

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Purification

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Effect on organism

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PTM

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Glycosylation

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Contaminant pathogens

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Commercialized products

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Table: Comparison of the different transgenic animal species used to produce recombinant enzymes.
Reference: Wang Y, Zhao S, Bai L, et al. BioMed research international, 2013, 2013.


With a long history of excel reputation, Creative Enzymes always fulfills various needs in expression and production of recombinant enzymes. Using an optimized transgenic animals expression system provides desired enzymes of consistent quality in batches. The whole process is realized through tightly controlled fermentation and purification, and stringent quality control is the bottom line for our global success and high levels of customer satisfaction. Creative Enzymes has been known as a leading company specialized in custom research in all types of enzyme related research specialized in enzyme expression and production. We have served thousands of customers from the pharmaceutical, biotechnological, and diagnostic industry, and are always open to new ideas and opportunities. Please contact us for any questions and inquries.



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