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IdeS protease

Official Full Name
IdeS protease
Background
IdeS protease digests IgG only at one specific site just below the hinge region, resulting in F(ab’)2and Fc-fragments. Since IdeS protease only cleaves at one specific site below the hinge region, there is no risk of over digestion if the incubation time is increased for several hours. IdeS protease digests IgG under physiological reaction conditions thus preserving the immunoreactivity. Best activity is obtained at 37°C but digestion can also be performed at room temperature using prolonged reaction time.
Synonyms
IdeS protease; FabRICATOR


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