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Biotinylated Luciferase from E. coli, Recombinant

Cat No.
NATE-1254
Description
Luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.
Abbr
Biotinylated Luciferase, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Appearance
Liquid form
Enzyme Commission Number
EC 1.13.12.7
Activity
> 1.0 x 10^10 relative light units (RLU)/ml Specific activity: 1.9 x 10^11 RLU/mg purified protein
Molecular Weight
ca. 70 kDa
pH Stability
5.5–9.0
Michaelis Constant
1.7 x 10^-4 M (ATP) 1.3 x 10^-4 M (D-luciferin)
Structure
monomer of ca. 70 kDa (SDS-PAGE)
Unit Definition
One relative light unit (RLU) is defined as the amount of enzyme which emits 1 count of light for 20 s at 30°C and pH 7.8.
Optimum pH
7.5–8.5
Thermal stability
below ca. 45°C
Stability
stable at 25°C for at least one week (liquid form)
Storage
at -20°C
Synonyms
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing); firefly luciferase; luciferase (firefly luciferin); Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing); firefly luciferin luciferase; Photinus pyralis luciferase; EC 1.13.12.7; 61970-00-1
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0422
EC No.EC 1.13.12.7
CAS No.9014-00-0
SourcePhotinus pyralis (firefly)
CatalogNATE-0423
EC No.EC 1.13.12.7
CAS No.9014-00-0
SourceVibrio fischeri (Photobacterium f)
CatalogNATE-0424
EC No.EC 1.13.12.7
CAS No.61970-00-1
SourceE. coli
CatalogNATE-1253
EC No.EC 1.13.12.7
CAS No.
SourceE. coli
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Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogCSUB-0354
ProductNameD(-)-Luciferin
EC No.
CAS No.2591-17-5
Source
CatalogCSUB-0355
ProductNameD-Luciferin
EC No.219-981-3
CAS No.2591-17-5
Source
CatalogCSUB-0356
EC No.
CAS No.103404-75-7
Source
CatalogCSUB-0357
ProductNameD-Luciferin
EC No.219-981-3
CAS No.2591-17-5
Source
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