Nanozymes-Based Protein Detection Services

Traditional methods of protein detection include high-performance liquid chromatography, capillary electrophoresis, gas chromatography, enzyme-linked immunoassay, electrochemical immunoassay, etc. These detection methods can achieve sensitive and accurate protein detection, but their disadvantages are also evident, such as expensive instruments, time-consuming and complex operations, and the need for professional operators. Therefore, nanozymes-based protein detection devices are considered as one of the potential methods due to their extremely high economy, convenient operation and fast detection.

Creative Enzymes offers comprehensive services covering a wide range of cutting-edge technologies to advance your nanozymes projects. Our scientists have developed a range of high-performance assays and innovative solutions for the detection of protein types. Armed with those unique solutions, detection will become easier and less error-prone.

Detection of Carcinoembryonic Antigen with Nanozymes

• Fe₃O₄ nanozymes always serve as promising HRP mimics for the immunodetection of antigens, antibodies, etc. Compared to conventional ELISA, this Fe₃O₄-based immunoassay is much easier, faster, cost-effective and sensitive. Inspired by this work, Creative Enzymes uses Fe₃O₄ nanozymes to detect carcinoembryonic antigen.
• Gold nanozymes have good biocompatibility. After loading the gold nanozymes, both the active site and the transfer rate increased. Based on this, we construct an electrochemical immunosensor for the detection of the carcinoembryonic antigen.
• According to the principle that the change of energy gap and contact resistance between carbon nanotubes lead to the change of electrical conductivity of the whole network of carbon nanotubes, we make a simple and efficient biosensor, and use this sensor to detect carcinoembryonic antigen.

Detection of Acetylcholinesterase with Nanozymes

• To quantitatively detect Acetylcholinesterase (AChE) activity, Creative Enzymes designs a novel colorimetric sensing platform based on MnO₂ nanozymes, which have the intrinsic peroxin-like properties. Acetyl cholinergic hydrolysis of acetylthiocholine to thiocholine products can decompose MnO₂ nanozymes, resulting in the degradation of the catalytic performance of MnO₂ nanozymes. Based on this, the activity of AChE can be measured by monitoring the oxTMB absorbance variations.

Fig. 1 MnO₂-based colorimetric probe for the detection of AChE activity.

• We design a simple, rapid and sensitive fluorescent biosensing method for the detection of acetylcholinesterase activity based on copper nanozymes, which are synthesized from polyT single-stranded DNA as a template.

Detection of Troponin I with Nanozymes

Using carboxylated Fe₃O₄ nanozymes as signal markers, we establish a novel nano mase paper chip (NIPC) with both visual interpretation and highly sensitive quantification for rapid detection of troponin I (cTnI). It provides a sensitive, rapid, qualitative and quantitative method for clinical detection.

Detection of Alpha Fetoprotein with Nanozymes

Based on the complementary pairing principle of aptamer base, we construct a silver nanozyme trimer surface-enhanced Raman sensor to detect alpha fetoprotein (AFP) in serum. In addition, the sensor is expected to serve as a universal detection platform for quantitative detection of other biomarkers.

Creative Enzymes has extensive experience and expertise in nanozyme services. We are committed to providing you with timely, quality deliverables. At the same time, we guarantee the cost-effectiveness, completeness and simplicity of the report. If you are interested in our services, please contact us for more details or make an online inquiry.