Detection of Hydrogen Peroxide with Nanozymes

Hydrogen peroxide (H₂O₂) is an important member of the reactive oxygen species, playing various roles in biology and medicine. Conventional detection methods for H₂O₂ are often restricted by their limited sensitivity, poor selectivity towards H₂O₂, inappropriate physicochemical properties for detection in biological environments, long response time, etc. Recently, nanozymes have been successfully used to detect hydrogen peroxide.

Creative Enzymes provides services to customers worldwide in the field of hydrogen peroxide detection based on nanozymes. Our platform is equipped with advanced facilities and professional experts to support nanozyme research. Here we provide various services according to customer needs. We deliver reliable results and reports on time to our customers around the world.

Detection of H₂O₂ with Ag@Fe₃O₄ Nanozymes

• Firstly, we prepare a magnetic nanozymes Ag@Fe₃O₄ with Ag core and Fe₃O₄ coated in its outer layer. We prepare them by a one-step solvothermal method.
• The Ag core is oxidized to Ag⁺ by H₂O₂, which causes the change of absorbance value of absorption spectrum. Based on this, Creative Enzymes establishes a rapid spectrophotometric method for the detection of hydrogen peroxide.
• The method can be used to detect hydrogen peroxide in tap water samples, and the recovery rate of nanozymes is higher.

Detection of H₂O₂ with DNA-Ag Nanozymes

• Under the protection of DNA, we synthesize DNA-Ag nanozymes by reducing silver nitrate with sodium borohydride.

Fig. 1 Detection of H₂O₂ by label-free colorimetric assay.

• The color of the solution change from yellowish brown to colorless after adding hydrogen peroxide to the nanozymes, therefore, Creative Enzymes establishes a colorimetric method to detect hydrogen peroxide.
• The method provided by us realizes the rapid and visual detection of hydrogen peroxide in food, improves the accuracy and sensitivity of detection, and provides a reference for the construction of detection methods for target objects in food.

Detection of H₂O₂ with Ni-MOF Nanozymes

• Firstly, we synthesize Ni-based organic skeleton (MOF) nanozymes by solvothermal method.
• In the presence of H₂O₂, Ni-MOF nanozymes can catalyze TMB to produce color reaction, which proves that Ni-MOF nanozymes have the properties of mimicking peroxidase. In addition, the affinity of Ni-MOF nanozymes for TMB and H₂O₂ substrates was higher than that of natural horseradish peroxidase (HRP). Based on this principle, Creative Enzymes applies it to label-free fluorescence detection of hydrogen peroxide.

Detection of H₂O₂ with FeVO₄ Nanozymes

• Using NH₄VO₃ and Fe (NO₃)₃· 9H₂O as raw materials, we use the hydrothermal synthesis method to prepare FeVO₄ nanozymes by adjusting the reaction system.
• In the presence of H₂O₂, FeVO₄ nanozymes can efficiently catalyze the oxidation of TMB to produce a blue oxide with a characteristic absorption peak at 652 nm. Based on this principle, Creative Enzymes applies it to label-free fluorescence detection of hydrogen peroxide.
• As a biocatalyst, they can be used in the detection of hydrogen peroxide concentration, biomedical analysis and residual hydrogen peroxide wastewater treatment.

Creative Enzymes provides cost-effective, high quality and comprehensive services to our clients worldwide. We provide our clients with direct access to our experts as well as prompt response to their inquiries. If you are interested in our services, please contact us for more details or make an online inquiry.