Nanozymes for Live Cell and Organelle Imaging

Cytology is an important method for the diagnosis of clinical diseases. Currently, the most commonly used cytological tests are flow cytometry, cytological smear and nucleic acid detection. These traditional methods are characterized by high technical requirements, time-consuming or costly. However, chromogenic reactions driven by nanozymes can be used for qualitative and quantitative analysis of cytological features.

Nanozymes methods exhibit a broader promise for imaging of living cells and organelles, as enzyme assays are faster, more cost-effective, and easier to operate. Creative Enzymes provides a variety of nanozymes for the imaging of living cell and organelle types to improve healthcare using technology.

Imaging of Circulating Tumor Cells with Nanozymes

• Circulating tumor cells (CTCS) are promising biomarkers for monitoring cancer metastasis and response to therapy.

Fig. 1 Imaging of circulating tumor cells based on nanozymes.

• To allow simple and rapid CTC detection, Creative Enzymes proposes a method based on Fe₃O₄ nanozymes, to achieve rapid separation of CTCS as well as nanozyme activity.
• The nanozyme can catalyze the oxidation of the colorimetric substrate TMB into blue product and realize visualization of CTCS. In addition, the visualized CTCS can be further quantified by UV-VIS measurements.

Imaging of Breast Cancer Cells with Nanozymes

• Breast cancer is the most common malignancy and also the second leading cause of cancer mortality in women globally.
• We develop an efficient method by detection of HER2+ breast cancer cells using an amplified signal scheme.

Fig. 2 Imaging of breast cancer cells based on nanozymes.

• The utilization of MSNs as an excellent enzyme immobilization support and gold nanoclusters as an effective peroxidase mimic imparts high sensitivity to this detection platform.

Imaging of Mitochondria in Living Cells with Nanozymes

• In addition to detecting CTCs, it is reported that researchers also employed the catalytic activity of nanozymes to design real-time detection probes for organelle imaging in living cells.
• We construct a versatile light-controlled biorthogonal catalyst based on palladium nanozymes, which can effectively realize the specific imaging of mitochondria in living cells. Its catalytic activity can be regulated by light-induced structural changes, mimicking the allosteric regulation mechanism of biological enzymes.

Creative Enzymes provides customized nano-zymes for imaging living cells and organelles. We are pleased to share our cutting-edge technology and extensive expertise to facilitate our clients' research and project development. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.