Serine-threonine protein kinase:these kinases appear to be involved in neurotransmitter release, control of stimulus-induced gene expression, and in the phosphorylation of microtubule related proteins.
CaMKII, Recombinant (Rat)
Calmodulin-Dependent Protein Kinase II (CaMKII) is a serine/threonine kinase. It is a Ca2+/calmodulin-dependent, truncated monomer (1-325 amino acid residues) of the α subunit. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CaMKII and produces substantial Ca2+/calmodulin-independent activity.
100 mM NaCl, 50 mM HEPES (pH 7.5 25°C), 0.1 mM EDTA, 1 mM DTT, 0.01% Brij 35 and 50% glycerol.
Apparent: 33 kDa Theoretical: 36 kDa
> 95% determined by SDS-PAGE
One unit is defined as the amount of activated CaMKII required to catalyze the transfer of 1 pmol of phosphate from ATP(200mM) to Autocamtide-2 (CaMKII Peptide Substrate), KKALRRQETVDAL (50 µM), in 1 minute at 30°C in a total reaction volume of 30 µl.
at -70°C. Avoid repeated freeze/thaw cycles.
Calmodulin-dependent Protein Kinase II; 9026-43-1