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Hexokinase-2 from Human, Recombinant

Cat No.
NATE-0845
Description
A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.
Abbr
HK2, Recombinant (Human)
Alias
HK2; HKII; HXK2
Source
E. coli
Species
Human
Appearance
Sterile filtered colorless solution.
Product Overview
HK2 Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain fused to His tag at the N-terminal encoding the sequence of 937 amino acids and having a molecular mass of 104.1 kDa. HXK2 is purified by proprietary chromatographic techniques.
Enzyme Commission Number
EC 2.7.1.1
Activity
3-4 units/ml
CAS No.
9001-51-8
Molecular Weight
104.1 kDa
Purity
Greater than 90.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Unit Definition
One unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30°C.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Buffer
The protein (1mg/ml) contains 20mM Tris pH8.0 and 10% glycerol.
Synonyms
hexokinase type IV glucokinase; hexokinase D; hexokinase type IV; hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; hexokinase; ATP:D-hexose 6-phosphotransferase; EC 2.7.1.1; 9001-51-8
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-202
EC No.EC 2.7.1.1
CAS No.9001-51-8
SourceMicroorganism
CatalogNATE-0342
EC No.EC 2.7.1.1
CAS No.9001-51-8
SourceSaccharomyces cerevisiae
CatalogNATE-0844
EC No.EC 2.7.1.1
CAS No.9001-51-8
SourceE. coli
CatalogNATE-0846
EC No.EC 2.7.1.1
CAS No.9001-51-8
SourceE. coli
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Realted Protocols
HEXOKINASE -Enzymatic Assay Protocol
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