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Native Abalone Sulfatase

Cat No.
NATE-0685
Description
Sulfatases EC 3.1.6.1 are enzymes of the esterase class that catalyze the hydrolysis of sulfate esters. These may be found on a range of substrates, including steroids, carbohydrates and proteins. Sulfate esters may be formed from various alcohols and amines. In the latter case the resultant N-sulfates can also be termed sulfamates. Sulfatases play important roles in the cycling of sulfur in the environment, in the degradation of sulfated glycosaminoglycans and glycolipids in the lysosome, and in remodelling sulfated glycosaminoglycans in the extracellular space. Together with sulfotransferases, sulfatases form the major catalytic machinery for the synthesis and breakage of sulfate esters.
Abbr
Sulfatases, Native (Abalone)
Alias
sulfatase
Source
Abalone entrails
Species
Abalone
Applications
Sulfatase from abalone entrails has been used in a study to determine that human p-selectin glycoprotein ligand-1 interacts with the skin-ass ociated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. Sulfatase from abalone entrails has also been used in a study to investigate HPLC of sulfate and glutathione conjugates from hamster embryo fibroblasts.
Form
lyophilized powder
Enzyme Commission Number
EC 3.1.6.1
Activity
20-40 units/mg solid
CAS No.
9016-17-5
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37°C (30 min assay).
Storage
−20°C
Synonyms
EC 3.1.6.1; 9016-17-5; sulfatase; nitrocatechol sulfatase; phenolsulfatase; phenylsulfatase; p-nitrophenyl sulfatase; arylsulfohydrolase; 4-methylumbelliferyl sulfatase; estrogen sulfatase; arylsulfatase
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CAS No.9016-17-5
Source
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sulfatase -Enzymatic Assay Protocol
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