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Enzymes for Research, Diagnostic and Industrial Use

Native Bacillus sp. Leucine dehydrogenase

Cat No.
DIA-209
Description
In enzymology, a leucine dehydrogenase (EC 1.4.1.9) is an enzyme that catalyzes the chemical reaction: L-leucine + H2O + NAD+ ↔ 4-methyl-2-oxopentanoate + NH3 + NADH + H+. The 3 substrates of this enzyme are L-leucine, H2O, and NAD+, whereas its 4 products are 4-methyl-2-oxopentanoate, NH3, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. This enzyme participates in valine, leucine and isoleucine degradation and valine, leucine and isoleucine biosynthesis.
Abbr
Leucine dehydrogenase (Bacillus sp.)
Source
Bacillus sp.
Applications
This enzyme is useful for enzyme determination of L-leucine and the activity of leucine amino-peptidase.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 1.4.1.9
Activity
GradeⅡ 20U/mg-solid or more (containing approx. 70% of stabilizers)
CAS No.
9082-71-7
Contaminants
Leucylpeptide decomposing enzymes (Leu-Val) < 1.0×10⁻²% (Leu-Gly-Gly) < 1.0×10⁻²% NADH oxidase < 1.0×10⁻²%
Molecular Weight
245 kDa
pH Stability
pH 5.5-10.5 (25°C, 20hr)
Michaelis Constant
1.0×10⁻³M (L-Leucine), 3.9×10⁻⁴M (NAD⁺), 3.5×10⁻⁵M (NADH), 3.1×10⁻⁴M [α-Ketoisocaproate (α-KIC)], 2.0×10⁻¹M (NH₃)
Structure
6 subunits per mol of enzyme
Optimum pH
10.5-10.8 (L-Leu→α-KIC), 9.4 (α-KIC→L-Leu)
Optimum temperature
above 70°C
Thermal stability
below 60°C (pH 6.9, 10min)
Stability
Stable at-20°C for at least one year
Stabilizers
2-Mercaptoethanol, L-cysteine, dithiothreitol, ethylenediaminetetraacetate
Inhibitors
Na₂S, Hg⁺⁺, Cu⁺⁺, Co⁺⁺, Mg⁺⁺, p-chloromercuribenzoate
Synonyms
EC 1.4.1.9; Leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); L-leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); LeuDH
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1715
EC No.EC 1.4.1.9
CAS No.9082-71-7
SourceMicroorganism
CatalogNATE-1805
EC No.EC 1.4.1.9
CAS No.9082-71-7
SourceE. coli
CatalogEXWM-1463
EC No.EC 1.4.1.9
CAS No.9082-71-7
Source
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1905
EC No.EC 1.4.1.9
CAS No.9082-71-7
SourceBacillus stearothermophilus
Realted Protocols
leucine dehydrogenase -Enzymatic Assay Protocol
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