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Native Escherichia coli Thioredoxin Reductase

Cat No.
NATE-0718
Description
Thioredoxin reductase (TrxR) is an NADPH-dependent oxidoreductase containing one FAD per subunit that reduces the active site disulfide in oxidised thioredoxin (Trx). The molecular weight of the isozymes from mammalian sources vary between 55-67 kDa as compared with 35 kDa in prokaryotes, plants or yeast. The substrate specificity of the mammalian enzyme is much broader than the prokaryotic enzyme reducing both mammalian and E. coli thioredoxins as well as well as non-disulfide substrates such selenite, lipoic acids, lipid hydroperoxides and hydrogen peroxide.
Abbr
TrxR, Native (Escherichia coli)
Alias
TrxR
Source
Escherichia coli
Applications
Thioredoxin Reductase from Escherichia coli can be used in peroxidase-coupled thioredoxin system assay for assessing the peroxidase activitiy of Cys-based thiol peroxidases. The product was used for determining the enzymatic activity of His6-Ahp1p.
Form
ammonium sulfate suspension; Suspension in 3.6 M (NH4)2SO4 containing 30 mM potassium phosphate buffer, pH 7.5, and 2 mM EDTA.
Enzyme Commission Number
EC 1.8.1.9
Activity
>25 units/mg protein (Bradford)
CAS No.
9074-14-0
Unit Definition
One unit will cause an increase in absorbance of 1.0 at 412 nm (when measured in a coupled assay with E. coli thioredoxin and DTNB) per min per mL at pH 7.0 at 25°C.
Storage
2-8°C
Synonyms
NADP-thioredoxin reductase; NADPH-thioredoxin reductase; thioredoxin reductase (NADPH); NADPH2:oxidized thioredoxin oxidoreductase; thioredoxin-disulfide reductase; EC 1.8.1.9; TrxR
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0713
EC No.EC 1.8.1.9
CAS No.9074-14-0
SourceRat liver
CatalogNATE-0917
EC No.
CAS No.9074-14-0
SourceE. coli
CatalogEXWM-1653
EC No.EC 1.8.1.9
CAS No.9074-14-0
Source
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