The activity of glycerol kinase is found widely in nature. In microorganisms GK makes possible the utilization of glycerol as a carbon source. In mammals the enzyme represents a juncture of sugar and fat metabolism; The enzyme is important to the clinical chemist in the determination of glycerol. GK is also useful in the assay of glyceraldehydes and dihydroxyacetone following their quantitative reduction to glycerol with sodium borohydride.
GK, Native (Flavobacterium meningosepticum)
Useful for the measurement of Triglyceride.
White to light grayish white amorphous powder, lyophilized.
Enzyme Commission Number
More than 70 U/mg solid
Hexokinase < 0.05%; Catalase < 0.1%; ATPase < 0.01%; Myokinase < 0.05%
150 kDa (TSK G3000SWXL) 50 kDa (SDS‒PAGE)
Glycerol 8.8 × 10-5M ATP 3.0 × 10-5M
One unit is defined as the amount of enzyme which converts 1 μmole of glycerol to glycerol‒3‒phosphate per minute at 37°C under the conditions specified in the assay procedure.
Stable at 60°C and below
Storage at -20°C in the presence of a desiccant is recommended.
glycerokinase; GK; ATP: glycerol-3-phosphotransferase; glycerol kinase phosphorylating; glyceric kinase; EC 184.108.40.206