In enzymology, a ADP-Dependent Hexokinase (EC 18.104.22.168) is an enzyme that catalyzes the chemical reaction: D‒Glucose + ADP → D‒Glucose‒6‒phosphate + AMP.
Hexokinase (ADP-Dependent), Native (Thermococcus litoralis)
Useful for the enzymatic determination of 1,5 Anhydroglucitol.
White amorphous powder, lyophilized
Enzyme Commission Number
More than 25 U/mg solid
NADPH oxidase < 0.01%; ATPase < 0.01%
50 kDa (gel filtration) 50 kDa (SDS‒PAGE)
Glucose 0.4 mM (at 37°C) ADP 0.057 mM (at 37°C)
One unit is defined as the amount of enzyme which converts 1 μmole of D‒glucose to D‒Glucose‒6‒phosphate per minute at 37°C under the conditions specified in the assay procedure.
Stable at 95°C and below
Storage at －20°C in the presence of a desiccant is recommended.
ADP-dependent glucokinase; ADP-specific glucokinase; ADP:D-glucose 6-phosphotransferase; EC 22.214.171.124