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Native Horseradish Peroxidase

Cat No.
PHAM-231
Description
The enzyme horseradish peroxidase (HRP), found in horseradish, is used extensively in molecular biology applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. HRP is often used in conjugates (molecules that have been joined genetically or chemically) to determine the presence of a molecular target. For example, an antibody conjugated to HRP may be used to detect a small amount of a specific protein in a western blot. Here, the antibody provides the specificity to locate the protein of interest and the HRP enzyme, in the presence of a substrate, produces a detectable signal. Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry.
Abbr
HRP (Horseradish)
Alias
HRP
Source
Horseradish
Applications
Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Horseradish peroxidase, product P8250, has been used to study nonoral antigens in inflamed gingiva1 and Ebola virus glycoprotein toxicity.
Form
essentially salt-free, lyophilized powder
Enzyme Commission Number
EC 1.11.1.7
CAS No.
9003-99-0
Unit Definition
One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Storage
2-8°C
Synonyms
EC 1.11.1.7; HRP; peroxidase; Horseradish Peroxidase; lactoperoxidase; guaiacol peroxidase; plant peroxidase; Japanese radish peroxidase; horseradish peroxidase (HRP); soybean peroxidase (SBP); extensin peroxidase; heme peroxidase; oxyperoxidase; protoheme peroxidase; pyrocatechol peroxidase; scopoletin peroxidase; Coprinus cinereus peroxidase; Arthromyces ramosus peroxidase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0549
EC No.EC 1.11.1.7
CAS No.9003-99-0
SourceBjerkandera adusta
CatalogNATE-0550
EC No.EC 1.11.1.7
CAS No.
SourceHorseradish Roots
CatalogEXWM-0509
ProductNameperoxidase
EC No.EC 1.11.1.7
CAS No.9003-99-0
Source
Realted Service
Realted Protocols
MYELOPEROXIDASE -Enzymatic Assay Protocol
PEROXIDASE from Soybean -Enzymatic Assay Protocol
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