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Native Human Glutathione S-Transferase

Cat No.
NATE-0326
Description
Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.
Abbr
GST, Native (Human)
Alias
GST
Source
Human placenta
Species
Human
Form
Lyophilized powder containing Tris buffer salts, reduced glutathione and EDTA.
Enzyme Commission Number
EC 2.5.1.18
Activity
25-125 units/mg protein
CAS No.
50812-37-8
Unit Definition
One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.
Storage
−20°C
Warnings
Protein determined by biuret. Purified and assayed by a modification of the method of Simons and Vander Jagt. Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.
Pathway
Biological oxidations, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Drug metabolism-cytochrome P450, organism-specific biosystem; Drug metabolism-cytochrome P450, conserved biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Arachidonate Epoxygenase / Epoxide Hydrolase, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Diurnally regulated genes with circadian orthologs, organism-specific biosystem; Drug metabolism-cytochrome P450, organism-specific biosystem; Drug metabolism-cytochrome P450, conserved biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem
Function
glutathione transferase activity; transferase activity; JUN kinase binding; S-nitrosoglutathione binding; dinitrosyl-iron complex binding; drug binding; glutathione binding; glutathione transferase activity; glutathione transferase activity; kinase regulator activity; nitric oxide binding; protein binding; transferase activity
Synonyms
EC 2.5.1.18; glutathione S-transferase; glutathione S-alkyltransferase; glutathione S-aryltransferase; S-(hydroxyalkyl)glutathione lyase; glutathione S-aralkyltransferase; glutathione S-alkyl transferase; GST; 50812-37-8
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