In enzymology, a glucose 1-dehydrogenase (EC 188.8.131.52) is an enzyme that catalyzes the chemical reaction: beta-D-glucose + NAD(P)+ ↔ D-glucono-1,5-lactone + NAD(P)H + H+. The 3 substrates of this enzyme are beta-D-glucose, NAD+, and NADP+, whereas its 4 products are D-glucono-1,5-lactone, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Glucose Dehyrogenase (Microorganism)
This enzyme is useful for enzymatic determination of D-Glucose.
White amorphous powder, lyophilized
Enzyme Commission Number
GradeⅢ 250U/mg-solid or more
NADH oxidase < 1.0×10⁻³% α-Glucosidase < 1.0×10⁻³% Glucose-6-phosphate dehydrogenase < 1.0×10⁻³%
approx. 101 kDa (Gel filtration)
pH 6.0-7.5 (20°C, 16hr)
NAD⁺linked : 1.38×10⁻²M (D-Glucose) 3.09×10⁻⁴M (NAD⁺), NADP⁺linked : 1.25×10⁻²M (D-Glucose) 4.07×10⁻⁵M (NADP⁺)
Specific for ß-D,-Glucose or 2-Deoxy-glucose (Either NAD⁺ or NADP⁺ serves as coenzyme.)
45°C (15min-treatment with 50mM K-phosphate buffer, pH 7.0)
Stable at-20°C for at least one year
Ag⁺, Hg²⁺, Monoiodoacetate
Glucose Dehyrogenase; EC 184.108.40.206; beta-D-glucose: NAD(P)+ 1-oxidoreductase; D-glucose dehydrogenase (NAD(P)+)