The activity of glycerol kinase is found widely in nature. In microorganisms GK makes possible the utilization of glycerol as a carbon source. In mammals the enzyme represents a juncture of sugar and fat metabolism; The enzyme is important to the clinical chemist in the determination of glycerol. GK is also useful in the assay of glyceraldehydes and dihydroxyacetone following their quantitative reduction to glycerol with sodium borohydride.
This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis.
White amorphous powder, lyophilized
Enzyme Commission Number
GradeⅢ 30 U/mg-solid or more
Catalase < 1.0×10⁻¹% NADH oxidase < 1.0×10⁻³% Adenosine triphosphatase < 1.0×10⁻³%
approx. 220 kDa (by gel filtration)
pH 5.5-10.0 (25°C, 20hr)
9.4×10⁻⁵M (Glycerol), 1.3×10⁻⁵M (ATP), 2.1×10⁻³M (Dihydroxyacetone)
Four subunits of approx. 58,000
below 65°C (pH 7.5, 30min)
p-Chloromercuribenzoate, Hg⁺⁺, Ag⁺
glycerokinase; GK; ATP: glycerol-3-phosphotransferase; glycerol kinase phosphorylating; glyceric kinase; EC 18.104.22.168