Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2.
CHOD, Native (Nocardia erythropolis)
in 1 M ammonium sulfate solution, pH 6, solution (slightly hazy)
Enzyme Commission Number
1 U corresponds to the amount of enzyme which converts 1 μmol cholesterol to 4-cholesten-3-one per minute at pH 7.5 and 25°C
Stable at –20°C for at least one year
EC 220.127.116.11; CHOD; cholesterol-O2 oxidoreductase; 3β-hydroxy steroid oxidoreductase; 3β-hydroxysteroid:oxygen oxidoreductase; 9028-76-6