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Enzymes for Research, Diagnostic and Industrial Use

Native Porcine Trehalase

Cat No.
NATE-0717
Description
Trehalase is a glycoside hydrolase enzyme located in on the brush border of the small intestine that catalyzes the conversion of trehalose to glucose. It is found in most animals. The non-reducing disaccharide trehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is one of the most important storage carbohydRates, which is present in almost all forms of life except mammals. The disaccharide is hydrolyzed into two molecules of glucose by the enzyme trehalase. There are two types of trehalases found in Saccharomyces cerevisiae, viz. neutral trehalase (NT) and acid trehalase (AT) classified according to their pH optima. NT has an optimum pH of 7.0, while that of AT is 4.5. Recently it has been reported that more than 90% of total AT activity in S. cerevisiae is extracellular and cleaves extracellular trehalose into glucose in the periplasmic space.
Abbr
Trehalase, Native (Porcine)
Alias
Trehalase
Source
Porcine kidney
Species
Porcine
Applications
Trehalase has been used in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves. Trehalase has also been used in a study to investigate growth arrest by trehalose-6-phosphate.
Product Overview
Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.
Form
buffered aqueous glycerol solution; Solution in 50% glycerol containing 1% Triton™ X-100 and 25 mM potassium phosphate, pH 6.5
Enzyme Commission Number
EC 3.2.1.28
Activity
> 1.0 units/mg protein
CAS No.
9025-52-9
Concentration
0.5-10.0 mg/mL protein basis (BCA)
Unit Definition
One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37°C (liberated glucose determined at pH 7.5).
Storage
−20°C
Synonyms
α,α-Trehalose glucohydrolase; Trehalase; EC 3.2.1.28; 9025-52-9; α,α-trehalase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1126
EC No.EC 3.2.1.28
CAS No.9025-52-9
SourceProkaryote
CatalogEXWM-3892
ProductNameα,α-trehalase
EC No.EC 3.2.1.28
CAS No.9025-52-9
Source
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Realted Protocols
trehalase -Enzymatic Assay Protocol
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