Products

Enzymes for Research, Diagnostic and Industrial Use

Native Porcine Trypsin

Cat No.
NATE-0723
Description
Trypsin (EC 3.4.21.4) is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive protease trypsinogen. Trypsin cleaves peptide chains mainly at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. The process is commonly referred to as trypsin proteolysis or trypsinisation, and proteins that have been digested/treated with trypsin are said to have been trypsinized.
Abbr
Trypsin, Native (Porcine)
Alias
Trypsin
Source
Porcine pancreas
Species
Porcine
Applications
For use in immunohistochemical procedures to enhance staining and to unmask antigens after routine fixation and processing. For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Product Overview
Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity. Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.
Form
lyophilized powder
Enzyme Commission Number
EC 3.4.21.4
Activity
Type II-S, 1 ,000-2 ,000 units/mg dry solid; Type IX-S, 13 ,000-20 ,000 BAEE units/mg protein
CAS No.
9002-07-7
Molecular Weight
23.8 kDa
Stability
Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at-20°C. The presence of Ca2+ will also diminish the self-autolysis of trypsin and maintain its stability in solution. Trypsin will also retain most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.
Storage
2-8°C
Buffer
Solubilizing trypsin should be done with a buffered salt solution contaiing no Ca2+ or Mg2+. This product is a lyophilized powder soluble in Hank′s Balanced Salt Solution at 25 mg/mL.
Synonyms
α-trypsin; β-trypsin; cocoonase; parenzyme; parenzymol; tryptar; trypure; pseudotrypsin; tryptase; tripcellim; sperm receptor hydrolase; Alpha-trypsin; Beta-trypsin; EC 3.4.21.4; Trypsin
Download Datasheet:


“Trypsin” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1867
EC No.EC 3.4.21.4
CAS No.
SourceBovine pancreas
CatalogNATE-1863
EC No.EC 3.4.21.4
CAS No.9002-07-7
SourceE. Coli
CatalogNATE-1893
EC No.EC 3.4.21.4
CAS No.9002-07-7
SourceE. coli
CatalogNATE-0721
EC No.EC 3.4.21.4
CAS No.9002-07-7
SourceBovine pancreas
+ See More >>
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogCEI-0824
EC No.
CAS No.9035-81-8
Source
Realted Protocols
Trypsin TPCK/DITC glass -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: