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Native Pseudomonas fluorescens Cholesterol Esterase

Cat No.
NATE-0116
Description
Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. This monomeric glycoprotein may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis.
Abbr
CE, Native (Pseudomonas fluorescens)
Alias
CE
Source
Pseudomonas fluorescens
Applications
Cholesterol esterase from Pseudomonas fluorescens has been used in an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase. Cholesterol esterase from Pseudomonas fluorescens has also been used in a study to investigate the nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations. This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.
Product Overview
Protein determined by biuret.
Form
lyophilized powder.
Enzyme Commission Number
EC 3.1.1.13
Activity
> 10,000 units/g protein
CAS No.
9026-00-0
Unit Definition
One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37°C in the presence of taurocholate.
Storage
−20°C
Buffer
0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
Synonyms
cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; 9026-00-0; sterol esterase; CE
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1679
EC No.EC 3.1.1.13
CAS No.9026-00-0
SourceCandida Rugosa
CatalogDIA-133
EC No.EC 3.1.1.13
CAS No.9026-00-0
SourceSchizophyllum commune
CatalogDIA-134
EC No.EC 3.1.1.13
CAS No.9026-00-0
SourcePseudomonas sp.
CatalogDIA-135
EC No.EC 3.1.1.13
CAS No.9026-00-0
SourceMicroorganism
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Realted Protocols
CHOLESTEROL ESTERASE -Enzymatic Assay Protocol
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