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Native Pseudomonas sp. Cholesterol Esterase

Cat No.
DIA-134
Description
Sterol esterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is steryl-ester acylhydrolase. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Esterase (Pseudomonas sp.)
Source
Pseudomonas sp.
Applications
This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Appearance
Light brown amorphous powder, lyophilized
Enzyme Commission Number
EC 3.1.1.13
Activity
GradeIII 100U/mg-solid or more (containing approx. 40% of stabilizers)
CAS No.
9026-00-0
Contaminants
Catalase < 1.0×10⁻²%
Molecular Weight
approx. 300 kDa
Isoelectric point
5.9±0.1
pH Stability
pH 5.0-9.0 (25°C, 24hr)
Michaelis Constant
5.4×10⁻⁵M (Linoleate), 6.6×10⁻⁵M (Oleate), 3.7×10⁻⁵M (Linolenate), 1.5×10⁻⁴M (Palmitate), 1.2×10⁻⁴M (Myristate), 2.3×10⁻⁵M (Stearate)
Optimum pH
7.0-9.0
Optimum temperature
40°C
Thermal stability
below 55°C (pH 7.5, 10min)
Stability
Stable at-20°C for at least one year
Stabilizers
Mg⁺⁺, Na-cholate, bovine serum albumin
Inhibitors
Hg⁺⁺, Ag⁺, ionic detergents
Synonyms
cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; Sterol esterase
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1679
EC No.EC 3.1.1.13
CAS No.9026-00-0
SourceCandida Rugosa
CatalogDIA-133
EC No.EC 3.1.1.13
CAS No.9026-00-0
SourceSchizophyllum commune
CatalogDIA-135
EC No.EC 3.1.1.13
CAS No.9026-00-0
SourceMicroorganism
CatalogDIA-281
EC No.
CAS No.
SourcePseudomonas species
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