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Native Pseudomonas sp. Cholesterol Oxidase

Cat No.
DIA-136
Description
Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Oxidase (Pseudomonas sp.)
Source
Pseudomonas sp.
Appearance
Yellowish amorphous powder, lyophilized
Product Overview
Recombinant Cholesterol Oxidase produced in E.coli has a molecular mass of about 34,000 Da.
Enzyme Commission Number
EC 1.1.3.6
Activity
Grade Ⅲ 15 U/mg-solid or more(contg. approx. 40% of stabilizers)
CAS No.
9026-00-0
Contaminants
Catalase < 1.0%; Cholesterol esterase < 1.0×10-2%
Molecular Weight
about 34 kDa (gel filtration)
Isoelectric point
5.1±0.1 and 5.4±0.1
pH Stability
pH 5.0~10.0 (25°C, 20hr)
Michaelis Constant
2.1×10-2 M (Creatine)
Structure
dimer
Optimum pH
6.5~7.0
Optimum temperature
45~50°C
Thermal stability
below 45°C (pH 7.0, 15min)
Stability
Stable at-20°C for at least 6 months
Inhibitors
onic detergents, Hg2+, Ag
Synonyms
Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase; EC 1.1.3.6
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-138
EC No.EC 1.1.3.6
CAS No.9028-76-6
SourceMicroorganism
CatalogDIA-284
EC No.
CAS No.
SourceBrevibacterium species
CatalogDIA-406
EC No.EC 1.1.3.6
CAS No.9028-76-6
SourceE. coli
CatalogDIA-408
EC No.EC 1.1.3.6
CAS No.9028-76-6
SourceE. coli
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