Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.
Cholesterol Oxidase (Pseudomonas sp.)
Yellowish amorphous powder, lyophilized
Recombinant Cholesterol Oxidase produced in E.coli has a molecular mass of about 34,000 Da.
Enzyme Commission Number
Grade Ⅲ 15 U/mg-solid or more(contg. approx. 40% of stabilizers)
Catalase < 1.0%; Cholesterol esterase < 1.0×10-2%
about 34 kDa (gel filtration)
5.1±0.1 and 5.4±0.1
pH 5.0~10.0 (25°C, 20hr)
2.1×10-2 M (Creatine)
below 45°C (pH 7.0, 15min)
Stable at-20°C for at least 6 months
onic detergents, Hg2＋, Ag
Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase; EC 188.8.131.52