Creatinine Amidohydrolase catalyzes the hydrolytic reaction converting creatinine to creatine. The enzyme is purified from a microorganism. The molecular size of the enzyme is approximately 175,000. The enzyme is useful for the enzy-matic assay of creatinine when coupled with other related enzymes. Creatinine + H2O → Creatine.
Creatinine amidohydrolase (Pseudomonas sp.)
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.
Lyophilized powder containing sucrose and BSA as stabilizers
Enzyme Commission Number
> 250U/mg protein
pH 7.5-9.0 (5°C, 16hr)
3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine)
6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
One unit will hydrolyze 1.0 mmole of creatinine to creatine per min at pH 8.0 and 25 °C
Below 70°C (pH 7.5, 30 min)
Ag+, Hg++, N-bromosuccinimide, EDTA
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides.
creatininase; creatinine hydrolase; creatinine amidohydrolase; EC 188.8.131.52; 9025-13-2