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Native Pseudomonas sp. Lipoprotein lipase

Cat No.
DIA-210
Description
Lipoprotein lipase (LPL) (EC 3.1.1.34) is a member of the lipase gene family, which includes pancreatic lipase, hepatic lipase, and endothelial lipase. It is a water soluble enzyme that hydrolyzes triglycerides in lipoproteins, such as those found in chylomicrons and very low-density lipoproteins (VLDL), into two free fatty acids and one monoacylglycerol molecule. It is also involved in promoting the cellular uptake of chylomicron remnants, cholesterol-rich lipoproteins, and free fatty acids. LPL requires ApoC-II as a cofactor.
Abbr
LPL (Pseudomonas sp.)
Alias
LPL
Source
Pseudomonas sp.
Applications
This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α-glycerophosphate oxidase and glycerol kinase. Usually, the reaction can be completed in 5 minutes at 37°C by using 2.5~3.0 units of the enzyme per test (3.0ml) at pH around 7.0.
Appearance
Light brown amorphous powder, lyophilized
Enzyme Commission Number
EC 3.1.1.34
Activity
GradeⅢ 20U/mg-solid or more (containing approx. 80% of stabilizers)
CAS No.
9004-02-8
Contaminants
Phosphatase < 1.0×10⁻³% Catalase < 2.0×10⁻²% NADH oxidase< 1.0×10⁻³% Cholesterol oxidase < 2.0×10⁻³%
Molecular Weight
approx. 134 kDa
Isoelectric point
5.95±0.05
pH Stability
pH 7.0-9.0 (25°C, 20hr)
Optimum pH
7.0-9.0
Optimum temperature
45-50°C
Thermal stability
below 55°C (pH 7.0, 10min)
Stability
Stable at-20°C for at least one year
Stabilizers
Mg⁺⁺, Na-cholate, bovine serum albumin
Inhibitors
Hg⁺⁺, Ag⁺, ionic detergents
Synonyms
Lipoprotein lipase; LPL; EC 3.1.1.34; Clearing factor lipase; Diacylglycerol lipase; Diglyceride lipase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-211
EC No.EC 3.1.1.34
CAS No.9004-02-8
SourceMicroorganism
CatalogDIA-282
EC No.
CAS No.
SourcePseudomonas species
CatalogNATE-0416
EC No.EC 3.1.1.34
CAS No.9004-02-8
SourceBovine milk
CatalogNATE-0417
EC No.EC 3.1.1.34
CAS No.9004-02-8
SourceBurkholderia sp.
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