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Native α-lytic protease

Cat No.
NATE-0052
Description
Alpha-lytic protease (aLP) is an alternative specificity protease for proteomics applications. This protease cleaves after T, A, S, and V residues. It geneRates peptides of similar average length as trypsin. aLP was first isolated from the myxobacterium Lysobacter enzymogenes. The pro-form of aLP is 397 amino acids long. In its mature form, aLP is 198 amino acids long. Its tertiary structural core resembles those of pancreatic serine proteases.
Abbr
α-LP, Native
Alias
α-LP
Form
Supplied as a solution in 10 mM sodium acetate buffer, pH 5.0.
Isoelectric point
9.69
Unit Definition
One unit will produce one mmole of p-nitroaniline per minute from N-succinyl-Ala-Ala-Ala-PNA at 25°C at pH 7.5
Optimum pH
5.0 (storage); 7.5 (activity)
Storage
−70°C
Synonyms
Alpha-lytic protease; myxobacter α-lytic proteinase; α-lytic proteinase; α-lytic protease; Mycobacterium sorangium α-lytic proteinase; Myxobacter 495 α-lytic proteinase; Alp
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