Products

Enzymes for Research, Diagnostic and Industrial Use

Protein Kinase Cη isozyme human, Recombinant

Cat No.
NATE-0576
Description
Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Abbr
PKCH, Recombinant (Human)
Alias
PRKCH; PKCH
Source
baculovirus infected insect cells
Species
Human
Product Overview
> 90% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution
Form
buffered aqueous glycerol solution
Enzyme Commission Number
EC 2.7.1.37
Molecular Weight
mol wt 82-84 kDa by SDS-PAGE
Purity
> 90% (SDS-PAGE)
Unit Definition
One unit will transfer 1 nmol of phosphate to PKC ε substrate peptide in 1 min at pH 7.4 at 30°C.
Storage
−70°C
Buffer
Solution in 20 mM HEPES, pH 7.4, 2 mM EDTA, 2 mM EGTA, 5 mM DTT, 250 mM NaCl, 0.05% Triton X-100, and 50% glycerol.
Pathway
Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Effects of PIP2 hydrolysis, organism-specific biosystem; Endothelins, organism-specific biosystem; G Protein Signaling Pathways, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; G alpha (z) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem
Function
ATP binding; enzyme binding; metal ion binding; nucleotide binding; protein kinase C activity
Synonyms
PRKCH; Ca2+-activated phospholipid-dependent serine-threonine kinase η isozyme human; PKCη human; PKCH; EC 2.7.1.37
Download Datasheet:


“PKC” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0573
EC No.EC 2.7.1.37
CAS No.141436-78-4
SourceRat brain
CatalogNATE-0574
EC No.
CAS No.
Sourcebaculovirus infected insect cells
CatalogNATE-0575
EC No.EC 2.7.1.37
CAS No.
Sourcebaculovirus infected insect cells
CatalogNATE-0577
EC No.
CAS No.
SourceE. coli
+ See More >>
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: