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Enzymes for Research, Diagnostic and Industrial Use

Sucrose Phosphorylase, Recombinant

Cat No.
NATE-0684
Description
Sucrose phosphorylase (E.C. 2.4.1.7) is an important enzyme in the metabolism of sucrose and regulation of other metabolic intermediates. Sucrose phosphorylase is in the class of hexosyltransferases. More specifically it has been placed in the retaining glycoside hydrolases family although it catalyzes a transglycosidation rather than hydrolysis. Sucrose phosphorylase catalyzes the conversion of sucrose to D-fructose and α-D-glucose-1-phosphate. It has been shown in multiple experiments that the enzyme catalyzes this conversion by a double displacement mechanism.
Abbr
Sucrose Phosphorylase, Recombinant
Source
E. coli
Applications
Sucrose phosphorylase has been used in a study to assess the enzymatic synthesis of stable, odorless, and powdered furanone glucosides. Sucrose phosphorylase has also been used in a study to investigate the novel transglucosylating reaction with carboxylic compounds.
Product Overview
Sucrose phosphorylase catalyzes the reversible conversion of sucrose (α-D-glucopyranosyl-1,2-β-D-fructofuranoside) and phosphate into D-fructose and α-D-glucose 1-phosphate.
Form
lyophilized powder; Contains sucrose as stabilizer.
Enzyme Commission Number
EC 2.4.1.7
Activity
> 45 units/mg solid
CAS No.
9074-06-0
Molecular Weight
mol wt 56 kDa by SDS-PAGE
Unit Definition
One unit will produce 1.0 μmole of D-fructose from sucrose per min with the corresponding reduction of NADP to NADPH at pH 7.6, at 25°C.
Storage
−20°C
Synonyms
Sucrose Phosphorylase; EC 2.4.1.7; 9074-06-0; sucrose glucosyltransferase; disaccharide glucosyltransferase; Sucrose:orthophosphate α-D-glucosytransferase
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0890
EC No.EC. 2.4.1.7
CAS No.9074-06-0
SourceLeuconostoc Mesenteroides
CatalogNATE-1532
EC No.EC. 2.4.1.7
CAS No.9074-06-0
SourceE. coli
CatalogEXWM-2613
EC No.EC 2.4.1.7
CAS No.9074-06-0
Source
Realted Service
Realted Protocols
SUCROSE PHOSPHORYLASE -Enzymatic Assay Protocol
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