Products

Enzymes for Research, Diagnostic and Industrial Use

Products
Online Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

24 hour
Promise

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.

Biotinylated Transglutaminase from Human, Proenzyme (Zymogen)

Cat No.
NATE-1735
Description
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor.
Abbr
TG 3, Recombinant (Human)
Alias
TG3
Source
E. coli
Species
Human
Applications
The transglutaminase 3 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. Biotinylated transglutaminase 3 may also be used for immunoprecipitation.
Appearance
Liquid
Package
100 µg
Form
The transglutaminase is formulated in 10 mM Sodium Phosphate pH 8.0, 15 mM NaCl.
Sample contains 50% glycerol. Transglutaminase is a Ca2+-dependent enzyme.
Enzyme Commission Number
EC 2.3.2.13
Activity
> 1000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after transglutaminase-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231)].
CAS No.
80146-85-6
Molecular Weight
78 kDa
Purity
> 95 % (visually by SDS-PAGE)
Unit Definition
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37 °C, assay volume = 1 ml).
Activators
10 μl His6-rhTG3 (1 μg/μl)
35 μl 50 mM Tris-HCl pH 8
5 μl Dispase I (Roche) (0.2 μg/μl)
Incubate for 20 min at 30 °C
Add 10 mM Ca2+ to activate transglutaminase3.
Storage
Store at -20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Synonyms
transglutaminase; EC 2.3.2.13; 80146-85-6; transglutaminase; Factor XIIIa; fibrinoligase; fibrin stabilizing factor; glutaminylpeptide γ-glutamyltransferase; polyamine transglutaminase; tissue transglutaminase; R-glutaminyl-peptide:amine γ-glutamyl transferase; protein-glutamine γ-glutamyltransferase; TG1

"Transglutaminase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1737 Transglutaminase 7 from Human, Recombinant EC 2.3.2.13 80146-85-6 E. coli Inquiry
NATE-1725 Biotinylated Transglutaminase from Human, Recombinant EC 2.3.2.13 80146-85-6 Insect cells Inquiry
NATE-1724 Transglutaminase 1 from Human keratinocyte, Recombinant EC 2.3.2.13 80146-85-6 Insect cells Inquiry
NATE-1723 Transglutaminase 1 from Human keratinocyte, Recombinant EC 2.3.2.13 80146-85-6 E. coli Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

0
Click unfold / close
Inquiry Basket
Delete selected Quote Check Out
Decide to move out of the shopping cart?
Sure No, Back

Please choose product!

< Go Back
You have already added to buy this product