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Human Sputum Elastase
Cat No.
CEFX-023
Description
1. Purulent human sputum fractionated with NaCl and treated with DNase.
2. Elastase is purified by affinity chromatography and separated from cathepsins proteins by ion exchange chromatography on carboxymethyl agarose.
3. Elastase is further purified by agarose-alanyl-alanyl-alanine affinity chromatography at -15°C.
4. The elastase is free of casein G activity as assayed by Suc-Ala-Ala-Pro-Phe-pNA.
2. Elastase is purified by affinity chromatography and separated from cathepsins proteins by ion exchange chromatography on carboxymethyl agarose.
3. Elastase is further purified by agarose-alanyl-alanyl-alanine affinity chromatography at -15°C.
4. The elastase is free of casein G activity as assayed by Suc-Ala-Ala-Pro-Phe-pNA.
Source
Leukocytes in septic human sputum
Species
Human
Form
Powder
Enzyme Commission Number
EC 3.4.21.37
Molecular Weight
29500
Storage
Store at 5°C. Stable for one year.
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