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Native Staphylococcus aureus Nuclease micrococcal

Cat No.
NATE-0452
Description
Micrococcal Nuclease is an endo-exonuclease that preferentially digests single-stranded nucleic acids. The rate of cleavage is 30 times greater at the 5' side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
Abbr
MNase, Native (Staphylococcus aureus)
Alias
MNase
Source
Staphylococcus aureus
Applications
Nuclease from Staphyl oc occus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.
Enzyme Commission Number
EC 3.1.31.1
Activity
100-300 units/mg protein
CAS No.
9013-53-0
Unit Definition
One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10 kDa for the mixed nucleotides.
Storage
−20°C
Warnings
One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37°C in a 3.55 mL reaction volume. Light path = 1 cm.
Synonyms
Micrococcal Nuclease; EC 3.1.31.1; spleen endonuclease; thermonuclease; nuclease T; micrococcal endonuclease; nuclease T'; staphylococcal nuclease; spleen phosphodiesterase; Staphylococcus aureus nuclease; Staphylococcus aureus nuclease B; ribonucleate (deoxynucleate) 3'-nucleotidohydrolase; 9013-53-0; Endonuclease micrococcal; MNase
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CatalogEXWM-3709
EC No.EC 3.1.31.1
CAS No.9013-53-0
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