Description
Alpha-lytic protease (aLP) is an alternative specificity protease for proteomics applications, whose wild-type (WT) version cleaves after T, A, S, and V residues. The M190A (Met190 → Ala190) mutant of aLP has different cleavage specificities, and cleaves after M, F, and L residues. Both the WT and M190A forms of aLP geneRate peptides of similar average length as trypsin.
Abbr
ALP M190A, Recombinant
Unit Definition
mmols of pNA produced per min per mg protein from 0.5 mM N-succinyl-Ala-Ala-Pro-Phe-PNA at 25°C in 100 mM Tris-HCl (pH 7.5).
Optimum pH
5.0(storage); 7.5(activity)
Synonyms
ALP M190A; Alpha-Lytic Protease M190A Mutant
