High-Throughput Adaptation

inquiry

Creative Enzymes provides specialized High-Throughput Adaptation Services to transform traditional enzymology assays into formats suitable for automated, large-scale screening. By combining robust assay design with advanced instrumentation, we deliver high-throughput solutions that maintain accuracy, reproducibility, and sensitivity while significantly accelerating data generation. Our services support applications ranging from drug discovery and functional genomics to enzyme engineering and industrial bioprocess optimization.

Understanding High-Throughput Enzymology Assays

The rapid pace of modern research and development demands efficient and scalable analytical methods. Conventional enzymology assays, while precise, are often too time-consuming for large-scale projects such as screening compound libraries, evaluating enzyme variants, or monitoring bioprocesses. High-throughput adaptation addresses this challenge by miniaturizing assays, automating workflows, and integrating advanced detection technologies.

High-throughput enzymology has become indispensable in fields such as:

Drug Discovery: Screening thousands of compounds against enzymatic targets.
Enzyme Engineering: Characterizing large panels of mutants for improved activity or stability.
Biotechnology and Industrial Applications: Monitoring enzyme performance across multiple conditions to optimize production efficiency.

High-throughput adaptation for enzymology assays

Creative Enzymes bridges the gap between traditional enzymology and high-throughput requirements by offering tailored adaptation services that deliver speed without compromising scientific rigor.

Our Service Offerings

Service Workflow

Workflow of high-throughput enzymology assay

Service Description

Our High-Throughput Adaptation Services encompass the design, optimization, and validation of enzyme assays for high-throughput platforms. We adapt existing assays or develop new ones that are compatible with automated liquid-handling systems, microplate readers, and robotics-based workflows.

Key Service Areas	Details
Miniaturization of Assays	Reducing reaction volumes to increase efficiency while maintaining data quality.
Automation Compatibility	Adapting assays for robotic systems and liquid-handling platforms.
Multiplexing	Designing assays that measure multiple parameters or enzyme activities in a single run.
High-Sensitivity Detection	Implementing fluorometric, luminescent, or absorbance-based methods suitable for low-volume reactions.
Throughput Optimization	Scaling up from hundreds to tens of thousands of data points per run.
Validation for Reproducibility	Ensuring robustness across plates, operators, and instruments.

Contact Our Team

Why Choose Creative Enzymes

Technical Expertise

A team of experienced enzymologists with proven success in high-throughput assay design.

Advanced Instrumentation

Access to automated platforms, high-resolution detectors, and microplate-based technologies.

Custom Adaptation

Tailored solutions for diverse assay types, from colorimetric to fluorometric and luminescent methods.

Scalability

Adaptation of assays for 96-, 384-, or 1536-well plate formats, depending on project requirements.

Time & Cost Efficiency

Faster data generation while minimizing reagent consumption and operational costs.

Global Trust

Widely recognized by pharmaceutical companies, research institutes, and industrial partners for reliable and scalable enzymology solutions.

Case Studies and Successes Stories

Case 1: Scaling a Kinase Assay to 384-Well High-Throughput Format

Client Challenge:

A pharmaceutical company's kinase inhibitor screening campaign was limited by a 96-well assay format. While reliable, the format required excessive reagent volumes, high costs, and weeks to complete primary screens of compound libraries.

Our Approach:

Miniaturized the assay to a 384-well plate format while maintaining signal robustness.
Optimized reagent concentrations and buffer conditions to minimize background interference.
Implemented automated liquid handling and integrated quality control checkpoints to ensure reproducibility.

Outcome:

Reduced reagent consumption by 65% and screening costs by 50%.
Increased throughput 4-fold, enabling the screening of 80,000 compounds in two weeks.
Achieved Z' factors >0.7, confirming excellent assay performance in the high-throughput format.

Case 2: High-Throughput Adaptation of Multiplex Enzyme Activity Assays for Food Enzyme Development

Client Challenge:

Global food ingredient company exploring novel enzyme blends for improved starch modification. The company initially measured amylase, glucoamylase, and pullulanase activities in separate low-throughput assays. This created long timelines for screening large enzyme libraries and hindered identification of synergistic enzyme combinations.

Our Approach:

We adapted the assays into a multiplex high-throughput workflow using 384-well plates and fluorescence-based detection. The method allowed simultaneous monitoring of multiple enzyme activities with automated liquid handling. Standard curves were recalibrated to account for overlapping signal ranges, ensuring accurate deconvolution of activity data.

Outcome:

Screening throughput increased from 50 to 2,000 samples per week.
Data analysis revealed two promising enzyme blends with 20% higher saccharification efficiency.
The company reduced development timelines by several months and filed a patent for their optimized enzyme cocktail.

FAQs

Q: What types of assays can be adapted for high-throughput use?

A: We adapt a wide variety of enzymatic assays, including spectrophotometric, fluorometric, and luminescent formats. Even assays with challenging substrates can be re-engineered for high-throughput compatibility.
Q: What throughput levels can Creative Enzymes support?

A: Our platforms support adaptation for 96- and 384-well plates, depending on project requirements and detection sensitivity.
Q: How do you ensure reproducibility in high-throughput formats?

A: All adapted assays undergo rigorous validation for inter-plate, inter-operator, and inter-day reproducibility. Statistical analysis ensures reliable performance across large data sets.
Q: Can you assist with both assay development and high-throughput implementation?

A: Yes. We provide end-to-end services, from assay design to adaptation and full validation on high-throughput platforms.

Name:

Email *

Phone:*

Company/Institution:

Country or Region:

Quantity:

Services & Products Interested *

Project Description:

Our Products Cannot Be Used As Medicines Directly For Personal Use.

Services

Online Inquiry