Detection of Dopamine with Nanozymes

Dopamine (DA) is a neurotransmitter that plays an important role in the mammalian central nervous system, kidney, hormone, and cardiovascular systems. Abnormal DA levels are associated with many diseases, so detection of DA in vitro and in vivo is becoming more and more important in clinical practice. In the past, various strategies for the determination of DA have been developed, assays based on nanozyme have attracted much attention due to their significant advantages, such as simple operation, low cost, rapid reaction, nondestructive testing, and easy miniaturization and automation.

Creative Enzymes makes full use of our specialized libraries, selection strategies and maturation techniques to optimize the stability and performance of nanozymes. With advanced technology and experienced staff, we provide a comprehensive set of nano-related services for global clients to support their research use.

Detection of Dopamine with Ni-Co Bimetals Nanozymes

• Creative Enzymes uses rGO, nickel nitrate, cobalt nitrate and TCPP as raw materials to prepare composite nanomaterials by solvothermal method.
• Specifically, we use reduced graphene oxide (rGO) as the base material and (Ni-Co) bimetal as the central atom to make electrochemical sensor modification material. With a large specific surface area, DA in the sample to be tested can be rapidly enriched to the electrode surface.
• Based on the principle, we prepare an electrochemical sensor with high sensitivity, high stability and good selectivity for the detection of DA.

Detection of Dopamine with Au-Ag Alloy Nanozymes

• Tb (Ⅲ) can coordinate with the catechol group of DA, and the concentration of DA can be determined by the change of the fluorescence intensity of Tb (Ⅲ) complex.
• We design Au-Ag alloy nanozymes to detect dopamine. On the one hand, sodium citrate acts as a protective agent and provides a suitable SEF distance between Au-Ag alloy nanozymes and Tb³⁺-DA complexes. On the other hand, by fixing Tb³⁺-DA, the collision between Tb³⁺ and water molecules is reduced, the non-radiative decay rate is reduced, and the Tb³⁺ fluorescence is enhanced.

Detection of Dopamine with Au Nanozymes

Fig. 1 A colorimetric assay based on iodine etched Au nanozymes are used for the determination of DA.

• Gold nanozymes possess tunable surface plasmon resonance (SPR) optical properties. In addition, I₂ generated by IO₃ reduction by dopamine etches Au nanozymes and blue-shifts their spectra.
• Based on the above principle, we develop a colorimetric analysis method which takes wavelength change as response signal. This method can be used for highly sensitive dopamine measurement.

Creative Enzymes offers professional services covering all glucose detection with enzymes to meet your research needs. With years of experience in nanozymes, our advanced platforms can help our clients solve various difficulties and conduct research. If you are interested in our services, please contact us for more details or make an online inquiry.